ErbB2 (pY1112) Antibody

ErbB2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors.

Target	ErbB2 (pY1112)
Clonality	Polyclonal
Target Modification	Tyr1112
Modification	Phosphorylation
Reactivity	Human
Tested Applications	ELISA, WB, DB
Host	Rabbit
Recommended dilutions	WB: 1/2000, DB: 1/500. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1112 of human ErbB2.
Isotype	IgG
Form	Liquid
Purification	Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P04626 (UniProt, ExPASy)
UniProt Secondary AC	B2RZG3, B4DHN3, Q14256, Q6LDV1, Q9UMK4, X5D2V5
UniProt Entry Name	ERBB2_HUMAN
KEGG	hsa:2064
String	9606.ENSP00000269571
Molecular Weight	Calculated MW: 138 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on ErbB2 (pY1112)

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