Flap Structure Specific Endonuclease 1 (FEN1) Antibody

FEN1 Antibody is a Rabbit Polyclonal antibody against FEN1. The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Target	Flap Structure Specific Endonuclease 1 (FEN1)
Clonality	Polyclonal
Reactivity	Human, Mouse
Tested Applications	WB, IHC, IF/ICC, IP
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/2000, IHC-P: 1/50 - 1/200, IF/ICC: 1/10 - 1/100, IP: 0.5-4 µg/200-400 µg whole cell extracts. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 50-380 of human FEN-1.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P39748 (UniProt, ExPASy)
UniProt Entry Name	FEN1_HUMAN
Gene Symbol	FEN1
GeneID	2237
NCBI Accession	NP_004102.1
KEGG	hsa:2237
String	9606.ENSP00000305480
Molecular Weight	Calculated MW: 43 kDa Observed MW: 48 kDa
Buffer	PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Flap Structure Specific Endonuclease 1 (FEN1)

Write a review

Your Name

Your Email Address

PMID

DOI

Your Review

Rating

Continue