+44 (0) 1223 755950
+1 832 327 7413
Human Fibronectin (FN1) Protein
REQUEST MORE INFO
Catalogue No: abx263226
Price:
US$275.50
(Size: 200 µg)
Orders to United States ship from United States
(
Click on the image to see the image legend
Add to Cart
Buy It Now
GET A QUOTE
Documents
Datasheet SDS
Human Fibronectin (FN1) Protein is a native protein isolated from Human plasma, provided as a white powder. Fibronectin (FN1) plays a role in several cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. It exists in two main forms: as an insoluble glycoprotein dimer that acts as a link in the extracellular matrix and as a soluble disulphide linked dimer found in the plasma. The plasma form is produced by hepatocytes, and the ECM form is synthesized by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin also takes part as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. Fibronectin organizes cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane-bound Fibronectin receptors on cell surfaces. Plasma fibronectin levels are elevated in severe coronary artery disease, and are linked to venous thromboembolism (VTE), particularly in males.
Research Articles on Fibronectin (FN1)
| Target | Fibronectin (FN1) |
| Research Area | Signal Transduction, Infection Immunity |
| Origin | Human |
| Expression | Native |
| Tested Applications | SDS-PAGE |
| Host | Human |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Not tested |
| Purity | ≥ 95% (SDS-PAGE) |
| Reconstitution | It is recommended to reconstitute with a chaotropic agent such as urea in sterile water at room temperature to a concentration of 0.2 mg/ml. Leave to stand for 1-2 hours. The recommended urea concentration is 4-5 M. Note: When using the protein as an attachment factor, wash the urea after attaching the fibronectin to the growth surface (plate or dish). |
| Storage | Stable at room temperature for up to 3 weeks. Store below -18°C for long-term storage. Upon reconstitution, store at 4°C for up to 1 week, or aliquot and store at or below -18°C for long term storage. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| Molecular Weight | 440 kDa. |
| Buffer | Prior to lyophilization: 10 mM sodium phosphate, pH 7.4, 0.15 M NaCl. |
| Concentration | Prior to lyophilization: 2 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |