Fluorescein Isothiocyanate (FITC) Antibody

Fluorescein Isothiocyanate (FITC) Antibody is a Goat Polyclonal antibody for the detection of FITC.

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.The antibody reacts with FITC but not the carrier protein.

Target	Fluorescein Isothiocyanate (FITC)
Clonality	Polyclonal
Reactivity	General (All species)
Tested Applications	ELISA
Host	Goat
Recommended dilutions	ELISA: 0.5-1 µg/ml. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	FITC conjugated to KLH
Isotype	IgG
Form	Liquid
Purity	≥ 95% (SDS-PAGE)
Purification	Purified by immunogen affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Validity	12 months.
Buffer	PBS, pH 7.4, containing 0.06% Proclin-300.
Concentration	2 mg/ml
Availability	Shipped within 5-12 working days.
Note	This product is for research use only.

Research Articles on Fluorescein Isothiocyanate (FITC)

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