Non-Esterified / Free Fatty Acids (NEFA) Assay Kit is an assay kit for the detection and quantification of Non-Esterified / Free Fatty Acids (NEFA) concentrations in tissue homogenates and cell lysates using a microplate reader at 715 nm.

NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.

Target	Non-Esterified / Free Fatty Acids (NEFA)
Research Area	Metabolic Diseases, Glycolysis And Lipid Metabolism, Energy Metabolism
Storage	Shipped at 4°C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Shelf Life	12 months.
Test Range	0.15 mmol/L - 1.5 mmol/L
Sensitivity	0.15 mmol/L
Detection Method	Colorimetric
Assay Data	Quantitative
Sample Type	Tissue homogenates and cell lysates.
Availability	Shipped within 5-10 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Research Articles on Non-Esterified / Free Fatty Acids (NEFA)

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