Green Fluorescent Protein (GFP) Antibody

Green Fluorescent Protein (GFP) Antibody is a Mouse Monoclonal antibody against GFP. GFP is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light.GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors.GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.GFP fluorescence is stable under fixation conditions and suitable for a variety of applications.GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Target	Green Fluorescent Protein (GFP)
Clonality	Monoclonal
Reactivity	General (All species)
Tested Applications	ELISA, IHC, FCM
Host	Mouse
Recommended dilutions	ELISA: 1/10000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Purified recombinant fragment of GFP expressed in E. coli.
Isotype	IgG2a
Form	Liquid
Purification	Purified from ascites by Protein G chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Molecular Weight	27 kDa
Buffer	PBS, containing 0.03% sodium azide.
Concentration	1 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Green Fluorescent Protein (GFP)

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