Green Fluorescent Protein (GFP) Antibody

GFP-Tag Antibody is a Mouse Monoclonal antibody against GFP-Tag. Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.

Target	Green Fluorescent Protein (GFP)
Clonality	Monoclonal
Reactivity	General (All species)
Tested Applications	WB, IF/ICC, IP
Host	Mouse
Recommended dilutions	WB: 1/2000 - 1/5000, IF/ICC: 1/50 - 1/100, IP: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 to the N-terminus of GFP protein.
Isotype	IgG1 Kappa
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Molecular Weight	Calculated MW: 27 kDa Observed MW: 27 kDa
Buffer	PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Green Fluorescent Protein (GFP)

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