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Green Fluorescent Protein (GFP) Antibody

Catalogue No: abx005585
Price: £150.00
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GFP-Tag Antibody is a Mouse Monoclonal antibody against GFP-Tag. Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.

Target Green Fluorescent Protein (GFP)
Clonality Monoclonal
Tested Applications WB, IF/ICC
Host Mouse
Recommended dilutions WB: 1/2000 - 1/3000, IF/ICC: 1/50 - 1/100. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Recombinant protein of GFP.
Isotype IgG
Purification Affinity purified.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentration > 1 mg/ml
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Research Articles on Green Fluorescent Protein (GFP) Antibody

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