Goat Anti-Human IgG Fc Antibody (HRP)
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Catalogue No: abx023551
Price:
US$1,073.00
(Size: 500 µl)
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Datasheet SDS
Goat Anti-Human IgG Fc Antibody (HRP) is a Goat Polyclonal antibody against Human IgG Fc. It is a polyclonal F (ab') 2 fragment to a human Immunoglobulin.
Research Articles on Human IgG Fc
| Target | Human IgG Fc |
| Clonality | Polyclonal |
| Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Host | Goat |
| Recommended dilutions | ELISA: 1/5000 - 1/100000, WB: 1/5000 - 1/200000. IHC: 1/500 - 1/5000. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | HRP |
| Form | Lyophilized |
| Purification | Purified from antisera by pepsin digestion and affinity chromatography. Fc fragments and whole IgG molecules have been removed. |
| Reconstitution | Reconstitute in 0.5 ml dH2O. Centrifuge if the solution is not completely clear after standing for 10 minutes at room temperature. |
| Storage | Store lyophilized at 2-8°C. After reconstitution, this product is stable for several weeks at 2-8°C as an undiluted liquid. Prepare working dilution only prior to immediate use. For long-term storage after reconstitution, add an equal volume of Glycerol to make a final concentration of 50% Glycerol and store at -20°C. |
| Shelf Life | At least 12 months. |
| Buffer | Prior to lyophilization: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.6 containing 15 mg/mL BSA. Buffer contains no preservative. |
| Specificity | Specific to the heavy chains on human IgG but not with the light chains on most human immunoglobulins. Minimal cross-reaction to Cow, Horse, and Mouse Serum Proteins. |
| Concentration | Prior to lyophilization: 0.8 mg/ml |
| Availability | Shipped within 10-20 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
| Directions for use | Use of NaN3 as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase. Prepare working dilution only prior to immediate use. |