Histone H1.0 (H1F0) Antibody
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Catalogue No: abx002346
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US$232.00
(Size: 20 µl)
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(![Western blot analysis of lysates from wild type (WT) and Histone H1.0 knockout (KO) 293T cells, using [KO Validated] Histone H1.0 Antibody at 1/1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 180s.](https://www.abbexa.com/image/cache/data/products/18B/abx002346_1-500x500.jpg "Histone H1.0 (H1F0) Antibody")
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Datasheet SDS
H1F0 Antibody is a Rabbit Polyclonal antibody against H1F0. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family.
Research Articles on Histone H1.0 (H1F0)
| Target | Histone H1.0 (H1F0) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC |
| Host | Rabbit |
| Recommended dilutions | ELISA: 1 µg/ml, WB: 1/500 - 1/1000, IHC-P: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Recombinant protein corresponding to H1F0. The exact sequence is proprietary. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P07305 (UniProt, ExPASy) |
| UniProt Secondary AC | B2R6I0, B4DRD6, Q6FG88, Q8N6R3 |
| UniProt Entry Name | H10_HUMAN |
| Gene Symbol | H1-0 |
| GeneID | 3005 |
| NCBI Accession | NP_005309.1 |
| KEGG | hsa:3005 |
| String | 9606.ENSP00000344504 |
| Molecular Weight | Calculated MW: 21 kDa Observed MW: 30 kDa |
| Buffer | PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol. |
| Concentration | > 0.2 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |