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| Target | Tyrosine Protein Kinase HCK Phospho-Tyr521 (HCK pY521) |
| Clonality | Polyclonal |
| Target Modification | Tyr521 |
| Modification | Phosphorylation |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB |
| Host | Rabbit |
| Recommended dilutions | ELISA: 1/10000, WB: 1/500 - 1/3000. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human HCK around the phosphorylation site of tyrosine 521. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P08631 (UniProt, ExPASy) |
| UniProt Entry Name | HCK_HUMAN |
| KEGG | hsa:3055 |
| String | 9606.ENSP00000444986 |
| Enzyme Commission Number | EC 2.7.10.2 |
| Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol. |
| Specificity | Detects endogenous levels of HCK only when phosphorylated at tyrosine 521. |
| Concentration | 1 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |