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Human High Mobility Group Protein B1 (HMGB1) Protein
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Catalogue No: abx261998
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US$261.00
(Size: 5 µg)
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Datasheet SDS
High Mobility Group Protein B1 (HMGB1) protein is a recombinant protein expressed in Hi-5 insect cells. It is a single, glycosylated polypeptide chain consisting of 223 amino acids. HMGB1 is a chromatin-binding protein found in eukaryotic cell nucleus and acts in the assembly of nucleoprotein complexes. Intracellularly, HMGB1 binds to DNA and is involved in transcriptional regulation. Outside the cell, it acts as a cytokine with activities that resemble those of tumor necrosis factor (TNF). It induces growth inhibition and apoptosis in macrophages through the RAGE intracellular signaling pathway, plays an important role in the relationship between necrosis and malignancy in glioma tumours and is also involved in Gram-negative sepsis by catalyzing movement of LPS monomers from LPS aggregates to CD14 to initiate a TLR4-mediated proinflammatory response. Overexpression of HMGB1 is common in gastrointestinal stromal tumors related to the KIT mutation, and is elevated significantly in chronic kidney disease patients, which correlates with glomerular filtration rate as well as with markers of inflammation and malnutrition.
Research Articles on High Mobility Group Protein B1 (HMGB1)
| Target | High Mobility Group Protein B1 (HMGB1) |
| Research Area | Infection Immunity |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | SDS-PAGE |
| Host | Insect |
| Conjugation | Unconjugated |
| Form | Liquid |
| Activity | Not tested |
| Purity | > 90% (SDS-PAGE) |
| Purification | Purified by proprietary chromatography techniques. Sterile filtered. |
| Storage | Store at 4°C for up to 4 weeks. Store at -20°C for long term storage. It is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P09429 (UniProt, ExPASy) |
| UniProt Secondary AC | A5D8W9, Q14321, Q5T7C3, Q6IBE1 |
| UniProt Entry Name | HMGB1_HUMAN |
| KEGG | hsa:3146 |
| String | 9606.ENSP00000345347 |
| Molecular Weight | 25 kDa |
| Sequence Fragment | 1-215 AA. |
| Sequence | MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH |
| Tag | C-terminal 8 His tag |
| Buffer | 20mM Tris-HCl, pH 8, containing 1 mM EDTA, 0.5 mM DTT and 10% glycerol. |
| Concentration | 1 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |