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Human High Mobility Group Protein B1 (HMGB1) Protein (Active)
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Catalogue No: abx651348
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US$899.00
(Size: 50 µg)
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Datasheet SDS
Human High Mobility Group Protein B1 (HMGB1) Protein (Active) is an active protein from Human. This protein exhibits binding activity as detailed in the Biological Activity section below. Other types of biological activity, such as enzymatic activity, have not been tested.
Research Articles on High Mobility Group Protein B1 (HMGB1)
| Target | High Mobility Group Protein B1 (HMGB1) |
| Research Area | Infection Immunity |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Active |
| Biological Activity | A binding ELISA assay was conducted to detect the interaction of recombinant Human HMGB1 and recombinant human SDF1. Briefly, HMG1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to SDF1-coated microplate wells and incubated for 2 h at 37 °C. Wells were washed with PBST and incubated for 1 h with anti-HMG1 polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of of HMG1 and SDF1 is shown in the figures. A dose-dependent effect was observed. |
| Purity | > 95% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P09429 (UniProt, ExPASy) |
| UniProt Entry Name | HMGB1_HUMAN |
| GabiPD GreenCard | HMGB1 |
| Gene Symbol | 3146 |
| GeneID | hsa:3146 |
| Ensembl | ENSG00000189403 |
| String | 9606.ENSP00000345347 |
| Molecular Weight | Calculated MW: 21.7 kDa Observed MW: 22 kDa |
| Sequence Fragment | Pro9-Arg163 |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300. |
| Endotoxin Level | < 1.0 EU/µg protein (LAL method) |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |