HindIII Enzyme

HindIII is expressed and purified from E. coli that carries the recombinant HindIII gene. The recognition site is A^AGCTT. The reaction is conducted for 5-10 minutes at 37 °C, and heat-inactivated at 65 °C for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Target	HindIII
Epitope Region	Recognition site: 5’...A↓AGCTT...3’ 3’...TTCGA↑A...5’
Form	Liquid
Storage	Store at -20°C. Avoid repeated freeze/thaw cycles.
Shelf Life	12 months.
Molecular Weight	35.8 kDa
Buffer	HindIII storage buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 µg/ml BSA, 50% Glycerol. 10x buffer: 500 mM Tris-Ac pH 7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA.
Concentration	20,000 units/ml
Availability	Shipped within 10-20 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. This product is shipped with dry ice.

Research Articles on HindIII

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