His Tag Antibody

His Tag Antibody is a Rabbit Polyclonal against His Tag. A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

Target	His Tag
Clonality	Polyclonal
Reactivity	General (All species)
Tested Applications	ELISA, WB
Host	Rabbit
Recommended dilutions	ELISA: 1/20000 - 1/80000, WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	A peptide corresponding to HHHHHH.
Isotype	IgG
Form	Lyophilized
Purification	Purified by antigen affinity column chromatography.
Reconstitution	Reconstitute in 100 µl of sterile distilled H2O with 50% glycerol.
Storage	Store at -20 °C. Avoid repeated freeze/thaw cycles.
Molecular Weight	Observed MW: 35 kDa
Buffer	Prior to lyophilization: 1% BSA and 0.02% NaN3.
Specificity	Detects His tag-fused proteins.
Concentration	Lyophilized form: Not applicable. After reconstitution: 1 mg/ml.
Availability	Shipped within 7-15 working days.
Note	This product is for research use only.

Research Articles on His Tag

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