His Tag Antibody
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Catalogue No: abx037944
Price:
US$398.75
(Size: 100 µg)
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Datasheet SDS
His Tag Antibody is a Rabbit Polyclonal against His Tag. A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
Research Articles on His Tag
| Target | His Tag |
| Clonality | Polyclonal |
| Reactivity | General (All species) |
| Tested Applications | ELISA, WB |
| Host | Rabbit |
| Recommended dilutions | ELISA: 1/20000 - 1/80000, WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | A peptide corresponding to HHHHHH. |
| Isotype | IgG |
| Form | Lyophilized |
| Purification | Purified by antigen affinity column chromatography. |
| Reconstitution | Reconstitute in 100 µl of sterile distilled H2O with 50% glycerol. |
| Storage | Store at -20°C. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| Molecular Weight | Observed MW: 35 kDa |
| Buffer | Prior to lyophilization: 1% BSA and 0.02% NaN3. |
| Specificity | Detects His tag-fused proteins. |
| Concentration | Lyophilized form: Not applicable. After reconstitution: 1 mg/ml. |
| Availability | Shipped within 7-15 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |