His Tag Antibody

His Tag Antibody is a Mouse Monoclonal against His Tag. A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

Target	His Tag
Clonality	Monoclonal
Reactivity	General (All species)
Tested Applications	WB, IF/ICC, IP
Host	Mouse
Recommended dilutions	WB: 1/2000 - 1/20000, IF/ICC: 1/50-1/200, IP: 1/50-1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	A synthetic peptide corresponding to His tag.
Isotype	IgG1
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Molecular Weight	Observed MW: 27 kDa, 35 kDa
Buffer	PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
Concentration	5.26 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on His Tag

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