Hoechst 33342 Staining Kit
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Catalogue No: abx097207
Price:
US$188.50
(Size: 1 Kit)
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Datasheet SDS
Hoechst 33342 Staining Kit contains Hoechst 33342 (Bisbenzimide) is a cell-permeable blue fluorescent stain that binds to adenine-thymine-rich regions of DNA, increasing its density. Kit components:
Research Articles on Hoechst 33342
- Hoechst 33342 Chromagen (5 mg/ml): 100 µl
- Dilution Buffer: 4 × 25 ml
| Target | Hoechst 33342 |
| Tested Applications | IF/ICC |
| Recommended dilutions | IF/ICC: 5 µg/ml (dye concentration). Optimal dilutions/concentrations should be determined by the end user. |
| Excitation/Emission | 355/465 |
| Storage | Store the Hoechst 33342 Chromogen at -20°C, and the dilution buffer at 4°C. Avoid exposure to light. |
| Shelf Life | Up to 12 months. |
| Buffer | Contains Hoechst 33342. |
| Availability | Shipped within 7-12 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
| Directions for use | For double or triple fluorescence staining in immunofluorescence tests, Hoechst 33342 staining is the last step after incubating the antibodies. For cultured cells, add 10 µl of Hoechst 33342 Chromogen to 2 ml Dilution Buffer in the same tube. Mix thoroughly (dye concentration is 5 μg/ml). Incubate for approximately 5 minutes in the dark at 30 °C, then wash with PBS/TBS 3 times, 3 minutes each time. |
