Homocysteine (Hcy) Assay Kit is an assay kit for the detection and quantification of Homocysteine (Hcy) concentrations in serum, plasma and urine using a microplate reader. Oxidized homocysteine is reduced to free homocysteine by triethyl phosphine (TCEP), and the free homocysteine reacts with the provided substrate to generate adenosine. The generated adenosine is immediately dehydrogenated into inosine and ammonia, and the ammonia further reacts with NADH under the catalysis of glutamate dehydrogenase to convert NADH to NAD+. The decrease in absorbance at 340 nm caused by the decline of NADH is proportional to the concentration of homocysteine in the sample.

Target	Homocysteine
Storage	Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity	The validity for this kit is 12 months.
Test Range	1.95 µmol/L - 100 µmol/L
Sensitivity	1.95 µmol/L
Detection Method	Colorimetric
Assay Data	Quantitative
Sample Type	Serum, plasma and urine.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Homocysteine

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