hTERT Antibody and Peptide Kit

The hTERT antibody is a sheep anti-human polyclonal which provides superior telomerase immunoprecipitation. This kit contains a solution of antigenic release peptide to facilitate dissociation of the hTERT antibody resulting in elution of telomerase after immunoprecipitation. The kit includes 100 µg of antibody and 125 µl of peptide.For more information please see the Nature Communications publication: Moye, A., Porter, K., Cohen, S. et al. Telomeric G-quadruplexes are a substrate and site of localization for human telomerase. Nat Commun 6, 7643 (2015). The working concentration for the antibody is approximately 20 µg of antibody per ml of lysate and for the peptide 25 µl peptide per 20 µg antibody (corresponding to 20 molar equivalents of peptide per antibody).

Notes: Based on immunoprecipitation of telomerase from HEK-293 tumour cell lysates, followed by a 1 hr elution in the presence of antigenic peptide, if available. There are no existing peptide antigens for the Calbiochem, Novus, Abcam, Rockland or Epitomics antibodies. Background activity in control represents non-specific adhesion to Protein G-Agarose beads.

Target	hTERT Antibody and Peptide Kit
Conjugation	Unconjugated
Storage	Aliquot and store at -20 °C or -80 °C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	O14746 (UniProt, ExPASy)
UniProt Secondary AC	O14783, Q2XS35, Q8N6C3, Q8NG38, Q8NG46
Gene Symbol	TERT
GeneID	7015
Buffer	Antibody: 10 mM HEPES-KOH (pH 8), 100 mM KCl, 0.05 w/v NaN3, 10% v/v glycerol. Peptide: H2O with 10% v/v glycerol + 0.05% w/v NaN3.
Concentration	Antibody: 1 mg/ml. Peptide: 100 µM of peptide in 150 µl aliquots.
Availability	Shipped within 1-3 working days.
Note	Abbexa's hTERT Antibody and Peptide Kit ("Licensed Products") may be subject of proprietary know how and one or more pending or granted patent applications, and are provided under license to Abbexa Ltd. The purchaser of Licensed Products is granted a non-exclusive, limited right to use the Licensed Products only for research purposes. Such license specifically excludes (i) the right to sell or otherwise transfer Licensed Products to third parties; (ii) use of Licensed Products for any diagnostic or therapeutic purpose; (iii) any use other than use in non-human animals or in vitro experiments. No right or license to perform commercial services of any kind using the Licensed Products, including without limitation reporting the results of recipient's activities for a fee or other commercial consideration, is hereby conveyed by the purchase of Licensed Products expressly, or by implication. Any use of Licensed Products other than for research purposes may require a licence. Please contact us for details.

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