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Human B-Cell Receptor CD22 (CD22) Protein (Biotin)
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Catalogue No: abx694333
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US$551.00
(Size: 20 µg)
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Human B-Cell Receptor CD22 (CD22) Protein (Biotin) is a recombinant Human protein containing a C-terminal His-AVI tag. Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Human Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. Two distinct human Siglec-2/CD22 cDNAs that arise from differential RNA processing of the same gene have been isolated. Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis.
Research Articles on B-Cell Receptor CD22 (CD22)
| Target | B-Cell Receptor CD22 (CD22) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | SDS-PAGE |
| Host | HEK293 cells |
| Conjugation | Biotin |
| Form | Lyophilized |
| Biological Activity | Binds to the Fc region of Human CD22 monoclonal antibody with an affinity constant of 0.16 nM when loaded on HIS1K Biosensor, as determined by BLI assay. |
| Purity | > 95% (SDS-PAGE) |
| Purification | 0.2 μm sterile filtered. |
| Reconstitution | Reconstitute in sterile H2O to prepare a stock solution of 0.25 mg/ml. Do not vortex. |
| Storage | Store lyophilized between -20 °C and -80 °C for up to 1 year. Reconstituted aliquots can be stored at 4-8°C for up to 1 week, or at < -20°C for up to 3 months. |
| Validity | The validity for this protein is 12 months. |
| UniProt Primary AC | P20273 (UniProt, ExPASy) |
| Molecular Weight | Calculated MW: 78 kDa Observed MW (SDS-PAGE): 110 kDa -130 kDa |
| Sequence Fragment | Asp20-Arg687 |
| Tag | C-terminal His tag and AVI tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4. |
| Endotoxin Level | < 1.0 EU per µg as determined by the LAL method. |
| Availability | Shipped within 5-15 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
| Directions for use | Centrifuge before opening to ensure complete recovery of vial contents. |