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Human Beta-Glucuronidase (GUSB) Protein
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Catalogue No: abx694171
Price:
US$536.50
(Size: 20 µg)
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Human Beta-Glucuronidase (GUSB) Protein is a recombinant Human protein expressed in HEK293 cells.Human beta-Glucuronidase (EC3.2.1.31) encoded by the GUSBgene is a lysosomal hydrolase involved in the degradation of glucuronic acid-containing glycosaminoglycans, including heparan sulfate, chondroitin sulfate and hyaluronan. The enzyme is only active on the glucuronic acid of the non-reducing end. The native protein is a tetrameric glycoprotein composed of identical subunits. Mutations in the GUSBgene are linked to mucopolysaccharidosis type VII- accumulation of partially degraded glycosaminoglycans with glucuronic acid residues at the non-reducing termini are usually found in the lysosomes of patients with the disease. This enzyme may contribute to the depletion of chondroitin from cartilage and can thereby facilitate the damage of joints in rheumatoid arthritis.
Research Articles on Human Beta-Glucuronidase (GUSB) Protein
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | SDS-PAGE |
| Host | HEK293 cells |
| Form | Lyophilized |
| Purity | >95% (SDS-PAGE) |
| Purification | 0.2 µm filtered. |
| Reconstitution | Reconstitute in sterile H2O to prepare a stock solution of 0.5 mg/ml. Do not vortex. |
| Storage | Store lyophilized between -20 °C and -80 °C for up to 1 year. |
| Stability | Stable when stored reconstituted at 2-8 °C for up to 1 week. Reconstituted aliquots are stable at -20 °C for up to 3 months. |
| UniProt Primary AC | P08236 (UniProt, ExPASy) |
| KEGG | hsa:299 |
| String | 966.ENSP32728 |
| Molecular Weight | Calculated MW: 71.5 kDa Observed MW (SDS-PAGE): 80 kDa |
| Sequence Fragment | Met1-Thr651 |
| Tag | C-terminal His tag |
| Buffer | Prior to lyophilization: PBS, containing 5% Trehalose and 5% Mannitol. |
| Activity | Not tested |
| Endotoxin Level | < 1.0 EU/mg (LAL method). |
| Availability | Shipped within 5-15 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
| Directions for use | Centrifuge before opening to ensure complete recovery of vial contents. |