Human Lys-63-Specific Deubiquitinase BRCC36 (BRCC3) Protein

Human Lys-63-Specific Deubiquitinase BRCC36 (BRCC3) Protein is a recombinant Human protein produced in a Prokaryotic expression system (E. coli). This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5.

Target	Lys-63-Specific Deubiquitinase BRCC36 (BRCC3)
Origin	Human
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 95%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P46736 (UniProt, ExPASy)
UniProt Secondary AC	A6QRF8, A6QRF9, A8MUX5, A8MWH0, A9Z1Y0, A9Z1Y5, B1B062, B4DQN7, Q16107, Q53YX5, Q9BTZ6
UniProt Entry Name	BRCC3_HUMAN
Gene Symbol	BRCC3
GeneID	79184
OMIM	300617
NCBI Accession	NP_001018065.1 NM_001018055.2 NP_001229569.1 NM_001242640.1 NP_077308.1 NM_024332.3
HGNC	24185
KEGG	hsa:79184
Ensembl	ENSG00000185515
String	9606.ENSP00000358474
Molecular Weight	Calculated MW: 19.8 kDa
Sequence Fragment	Gln7-Gly148
Tag	N-terminal His tag
Buffer	Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
Activity	Not tested
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on Lys-63-Specific Deubiquitinase BRCC36 (BRCC3)

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