Human Caldesmon (CALD1) Protein

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Catalogue No: abx065663
Price: US$246.50
(Size: 10 µg)

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Human Caldesmon (CALD1) Protein Human Caldesmon (CALD1) Protein

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Datasheet SDS
Human Caldesmon (CALD) is a recombinant Human protein expressed in E. coli.

Target Caldesmon (CALD1)
Research Area Metabolism, Developmental Science, Bone Metabolism
Origin Human
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Activity Not tested
Purity > 97%
Reconstitution To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.
Shelf Life 12 months.
UniProt Primary AC Q05682 (UniProt, ExPASy)
UniProt Secondary AC A8K0X1, Q13978, Q13979, Q14741, Q14742, Q9UD91
UniProt Entry Name CALD1_HUMAN
Gene Symbol CALD1
GeneID 800
OMIM 114213
NCBI Accession NP_004333.1 NM_004342.6 NP_149129.2 NM_033138.3
KEGG hsa:800
String 9606.ENSP00000354826
Molecular Weight Calculated MW: 25.1 kDa
Observed MW (SDS-PAGE): 38 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Gln28-Gln207
Sequence QRN DDDEEEAARE RRRRARQERL RQKQEEESLG QVTDQVEVNA QNSVPDEEAK TTTTNTQVEG DDEAAFLERL ARREERRQKR LQEALERQKE FDPTITDASL SLPSRRMQND TAENETTEKE EKSESRQERY EIEETETVTK SYQKNDWRDA EENKKEDKEK EEEEEEKPKR GSIGENQ
Tag N-terminal His tag
Buffer Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.
Concentration Prior to lyophilization: 120 µg/ml
Availability Shipped within 5-7 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Caldesmon (CALD1)


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