Human Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5) Protein (Active)
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Catalogue No: abx655572
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(Size: 50 µg)
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Datasheet SDS
Human CEACAM5 Protein (Active) is an Active recombinant Human protein expressed in 293F cells for use in cell culture and activity assays. This protein exhibits binding activity as detailed in the Biological Activity section below. Other types of biological activity, such as enzymatic activity, have not been tested.
Research Articles on Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5)
| Target | Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5) |
| Research Area | Tumor Immunity, Gastroenterology |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | 293F cells |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Active |
| Biological Activity | Carcinoembryonic Antigens (CEAs) are members of the CD66 cluster of differentiation. They are glycophosphatidylinositol (GPI)-anchored intercellular molecules that are upregulated in many cancers, such as colon, breast and lung. Galectin 4 (GAL4) has been identified as an interactor of CEA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CEA and recombinant human GAL4. Briefly, CEA was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to GAL4-coated microplate wells and incubated for 2 h at 37 °C. Wells were washed with PBST and incubated for 1 h with anti-CEA polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of CEA and GAL4 is shown in Figure 4. |
| Purity | > 98% |
| Reconstitution | Reconstitute in ddH2O to a concentration between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P06731 (UniProt, ExPASy) |
| UniProt Secondary AC | H9KVA7 |
| UniProt Entry Name | CEAM5_HUMAN |
| Gene Symbol | CEACAM5 |
| GeneID | 1048 |
| KEGG | hsa:1048 |
| String | 9606.ENSP00000221992 |
| Molecular Weight | Calculated MW: 73 kDa Observed MW (SDS-PAGE): 80 kDa |
| Sequence Fragment | Lys35-Ala685 |
| Sequence | KLTIES TPFNVAEGKE VLLLVHNLPQ HLFGYSWYKG ERVDGNRQII GYVIGTQQAT PGPAYSGREI IYPNASLLIQ NIIQNDTGFY TLHVIKSDLV NEEATGQFRV YPELPKPSIS SNNSKPVEDK DAVAFTCEPE TQDATYLWWV NNQSLPVSPR LQLSNGNRTL TLFNVTRNDT ASYKCETQNP VSARRSDSVI LNVLYGPDAP TISPLNTSYR SGENLNLSCH AASNPPAQYS WFVNGTFQQS TQELFIPNIT VNNSGSYTCQ AHNSDTGLNR TTVTTITVYA EPPKPFITSN NSNPVEDEDA VALTCEPEIQ NTTYLWWVNN QSLPVSPRLQ LSNDNRTLTL LSVTRNDVGP YECGIQNKLS VDHSDPVILN VLYGPDDPTI SPSYTYYRPG VNLSLSCHAA SNPPAQYSWL IDGNIQQHTQ ELFISNITEK NSGLYTCQAN NSASGHSRTT VKTITVSAEL PKPSISSNNS KPVEDKDAVA FTCEPEAQNT TYLWWVNGQS LPVSPRLQLS NGNRTLTLFN VTRNDARAYV CGIQNSVSAN RSDPVTLDVL YGPDTPIISP PDSSYLSGAN LNLSCHSASN PSPQYSWRIN GIPQQHTQVL FIAKITPNNN GTYACFVSNL ATGRNNSIVK SITVSASGTS PGLSA |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 10 mM PBS, pH 7.6, containing 5% Trehalose. |
| Endotoxin Level | < 1.0 EU/µg protein (LAL method) |
| Concentration | Prior to lyophilization: 350 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |