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Human Dihydroorotate Dehydrogenase (DHODH) Protein
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Catalogue No: abx655605
Price:
US$681.50
(Size: 50 µg)
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Human Dihydroorotate Dehydrogenase (DHODH) Protein (Active) is an Active Recombinant Human protein expressed in E. coli.
Research Articles on Dihydroorotate Dehydrogenase (DHODH)
| Target | Dihydroorotate Dehydrogenase (DHODH) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 92% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q02127 (UniProt, ExPASy) |
| UniProt Secondary AC | A8K8C8, Q6P176 |
| UniProt Entry Name | PYRD_HUMAN |
| Gene Symbol | DHODH |
| GeneID | 1723 |
| OMIM | 126064 |
| KEGG | hsa:1723 |
| String | 9606.ENSP00000219240 |
| Molecular Weight | Calculated MW: 42.4 kDa Observed MW (SDS-PAGE): 42 kDa |
| Sequence Fragment | Arg35-Asp392 |
| Sequence | RFYAEH LMPTLQGLLD PESAHRLAVR FTSLGLLPRA RFQDSDMLEV RVLGHKFRNP VGIAAGFDKH GEAVDGLYKM GFGFVEIGSV TPKPQEGNPR PRVFRLPEDQ AVINRYGFNS HGLSVVEHRL RARQQKQAKL TEDGLPLGVN LGKNKTSVDA AEDYAEGVRV LGPLADYLVV NVSSPNTAGL RSLQGKAELR RLLTKVLQER DGLRRVHRPA VLVKIAPDLT SQDKEDIASV VKELGIDGLI VTNTTVSRPA GLQGALRSET GGLSGKPLRD LSTQTIREMY ALTQGRVPII GVGGVSSGQD ALEKIRAGAS LVQLYTALTF WGPPVVGKVK RELEALLKEQ GFGGVTDAIG AD |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose. |
| Activity | Active |
| Biological Activity | Dihydroorotate Dehydrogenase (DHODH) is an enzyme which catalyzes the ubiquinone-mediated oxidation of dihydroorotate to orotate in de novo pyrimidine biosynthesis. It is a mitochondrial protein present on the outer surface of the inner mitochondrial membrane and is associated with the electron transport chain. FK506 Binding Protein 8 (FKBP8) has been identified as an interactor of DHODH, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DHODH and recombinant human FKBP8. Briefly, DHODH was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FKBP8-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-DHODH polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of DHODH and FKBP8 is shown in Figure 3. |
| Endotoxin Level | < 1.0 EU per µg as determined by the LAL method. |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
