Human Anti-glycyl tRNA Synthetase-Antibody / Anti-GlyRS-Antibody (anti-EJ) ELISA Kit

Catalogue No: abx351490
Price: £470.00
(Size: 96 tests)

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Human Anti-glycyl tRNA Synthetase-Antibody / Anti-GlyRS-Antibody (anti-EJ) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human EJ-Antibody concentrations in serum, plasma and other biological fluids.

Introduction Anti-glycyl-tRNA Synthetase (anti-EJ) has been associated with diseases such as interstitial lung disease, dermatomyositis, and polymyositis.
Target EJ-Antibody
Reactivity Human
Tested Applications ELISA
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Storage Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity The validity for this kit is 6 months.
Stability The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
UniProt Primary AC P41250 (UniProt, ExPASy)
KEGG hsa:2617
String 9606.ENSP00000373918
Test Range 1.56 ng/ml - 100 ng/ml
Sensitivity 0.94 ng/ml
Standard Form Lyophilized
Detection Method Colorimetric
Assay Type Indirect
Assay Data Quantitative
Sample Type Serum, plasma and other biological fluids.
Target Type Antigen
Kit Components The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
  • Pre-coated 96-Well Microplate
  • Standard
  • Standard Diluent Buffer
  • Wash Buffer
  • Detection Reagent A
  • Detection Reagent B
  • Diluent A
  • Diluent B
  • TMB Substrate
  • Stop Solution
  • Plate Sealer
Material Required But Not Provided
  • 37°C incubator
  • Multi and single channel pipettes and sterile pipette tips
  • Squirt bottle or automated microplate washer
  • 1.5 ml tubes
  • Distilled water
  • Absorbent filter papers
  • 100 ml and 1 liter graduated cylinders
  • Microplate reader (wavelength: 450 nm)
  • ELISA Shaker
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of EJ-Antibody were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of EJ-Antibody were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) × 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%
Availability Shipped within 5-7 working days.
Note This product is for research use only.

The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.

Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
Plate coated with Antigen
Research Articles on EJ-Antibody

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