Human Flagellin (FliC) ELISA Kit

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Catalogue No: abx576568
Price: US$717.75
(Size: 96 tests)

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Human Flagellin (FliC) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human Flagellin concentrations in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Introduction Flagellin is a globular protein that arranges itself in a hollow cylinder to form the filament in a bacterial flagellum. It has a mass of about 30 to 60 kDa. Flagellin is the principle component of bacterial flagellum, and is present in large amounts on nearly all flagellated bacteria. The structure of flagellin is responsible for the helical shape of the flagellar filament, which is important for its proper function. The N- and C-termini of flagellin form the inner core of the flagellin protein, and is responsible for flagellin's ability to polymerize into a filament. The middle residues make up the outer surface of the flagellar filament. While the termini of the protein are quite similar among all bacterial flagellins, the middle portion is wildly variable.
Target Flagellin (FliC)
Reactivity Human
Tested Applications ELISA
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Storage Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity The validity for this kit is at least 6 months. Up to 12 months validity can be provided on request.
Stability The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
UniProt Primary AC P04949 (UniProt, ExPASy)
Test Range 0.78 ng/ml - 50 ng/ml
Sensitivity < 0.28 ng/ml
Standard Form Lyophilized
Detection Method Colorimetric
Assay Type Sandwich
Assay Data Quantitative
Sample Type Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Availability Shipped within 5-12 working days.
Note This product is for research use only.

The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.

Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
Standard 50 ng/ml
Research Articles on Flagellin (FliC)


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