Human Glutamate Decarboxylase 2 (GAD2) Protein
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Catalogue No: abx066816
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US$275.50
(Size: 10 µg)
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Datasheet SDS
Human Glutamate Decarboxylase 2 (GAD2) is a recombinant Human protein expressed in E. coli. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
Research Articles on Glutamate Decarboxylase 2 (GAD2)
| Target | Glutamate Decarboxylase 2 (GAD2) |
| Research Area | Enzymes and Kinases, Endocrinology, Neuroscience, Autoimmunity |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Not tested |
| Purity | > 98% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | Q05329 (UniProt, ExPASy) |
| UniProt Secondary AC | Q9UD87 |
| UniProt Entry Name | DCE2_HUMAN |
| KEGG | hsa:2572 |
| String | 9606.ENSP00000365437 |
| Molecular Weight | Calculated MW: 34.4 kDa Observed MW: 34 kDa (determined by SDS-PAGE) |
| Sequence Fragment | Arg65-Arg105 |
| Sequence | RAAARK AACACDQKPC SCSKVDVNYA FLHATDLLPA CDGER |
| Tag | N-terminal His tag and GST tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300. |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |