Human Glutamate Decarboxylase 2 (GAD2) Protein

Recombinant Glutamate Decarboxylase 2 (GAD2) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli). This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Target	Glutamate Decarboxylase 2 (GAD2)
Origin	Human
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 98%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q05329 (UniProt, ExPASy)
UniProt Secondary AC	Q9UD87
UniProt Entry Name	DCE2_HUMAN
KEGG	hsa:2572
String	9606.ENSP00000365437
Molecular Weight	Calculated MW: 34.4 kDa Observed MW: 34 kDa (determined by SDS-PAGE)
Sequence Fragment	Arg65-Arg105
Sequence	RAAARK AACACDQKPC SCSKVDVNYA FLHATDLLPA CDGER
Tag	N-terminal His tag and GST tag
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.
Activity	Not tested
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on Glutamate Decarboxylase 2 (GAD2)

Write a review

Your Name

Your Email Address

PMID

DOI

Your Review

Rating

Continue