Human Cadherin-13 (CDH13) ELISA Kit

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Catalogue No: abx051689
Price: US$645.25
(Size: 96 tests)
Reviews: 1 Publications and Reviews
(1 Publications and Reviews)

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Graph showing standard OD data for Human Cadherin-13 (CDH13)
Human Cadherin-13 (CDH13) ELISA Kit

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Datasheet SDS Download Manual
Human Cadherin-13 (CDH13) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human Cadherin-13 (CDH13) concentrations in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Introduction T-cadherin also known as cadherin 13, H-cadherin (heart) (CDH13) is a unique member of cadherin superfamily because it lacks the transmembrane and cytoplasmic domains and is anchored to the cells membrane through the GPI anchor. Classical cadherins are necessary for cell–cell contacts, dynamic regulation of morphogenetic processes in embryos and tissue integrity in adult organism. Cadherins function as membrane receptors mediating outside-in signals, activating small GTPases and beta-catenin/Wnt pathway, and resulting in dynamic cytoskeleton reorganization and changes in the phenotype. T-cadherin is a GPIanchored member of cadherin superfamily, which lacks a direct contact with cytoskeleton and therefore is not involved in cell–cell adhesion. It is involved in low density lipoproteins (LDL) hormone-like effects on Ca2+-mobilization and increased cell migration as well as phenotype changes. Exact signaling partners and adapter proteins for T-cadherin remain to be elucidated.
Target Cadherin-13 (CDH13)
Reactivity Human
Tested Applications ELISA
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Storage Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity The validity for this kit is at least 6 months. Up to 12 months validity can be provided on request.
Stability The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
UniProt Primary AC P55290 (UniProt, ExPASy)
UniProt Secondary AC A8W476, A8W477, B7Z590, C9JRI6, J3KN62, Q6GTW4, Q8TBX3
UniProt Entry Name CAD13_HUMAN
Gene Symbol CDH13
GeneID 1012
OMIM 601364
HGNC 1753
KEGG hsa:1012
String 9606.ENSP00000268613
Test Range 31.2 pg/ml - 2000 pg/ml
Sensitivity 18.75 pg/ml
Standard Form Lyophilized
Detection Method Colorimetric
Assay Type Sandwich
Assay Data Quantitative
Sample Type Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Kit Components The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
  • Pre-coated 96-Well Microplate
  • Standard
  • Standard Diluent Buffer
  • Wash Buffer
  • Detection Reagent A
  • Detection Reagent B
  • Diluent A
  • Diluent B
  • TMB Substrate
  • Stop Solution
  • Plate Sealer
Material Required But Not Provided
  • 37°C incubator
  • Multi and single channel pipettes and sterile pipette tips
  • Squirt bottle or automated microplate washer
  • 1.5 ml tubes
  • Distilled water
  • Absorbent filter papers
  • 100 ml and 1 liter graduated cylinders
  • Microplate reader (wavelength: 450 nm)
  • ELISA Shaker
Assay Procedure This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
  • 1) Set standard, test samples and control wells.
  • 2) Aliquot 100 µl of diluted standard into the standard wells.
  • 3) Aliquot 100 µl of Standard Diluent buffer into control (zero) well.
  • 4) Aliquot 100 µl of diluted samples into the sample wells. Incubate for 1 hr at 37 °C.
  • 5) Aliquot 100 µl of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
  • 6) Wash 3 times.
  • 7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 90 mins at 37 °C.
  • 8) Wash 5 times.
  • 9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
  • 10) Aliquot 50 µl of Stop Solution.
  • 11) Measure the OD at 450 nm.
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Cadherin-13 (CDH13) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Cadherin-13 (CDH13) were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) × 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%
Availability Shipped within 5-7 working days.
Note This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit.If you require a specific range, please contact us in advance or write your request in your order comments.
Plate coated with Antibody
Research Articles on Cadherin-13 (CDH13)


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