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Human Heparanase (HPA) Protein (Active)
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Catalogue No: abx655871
Price:
US$739.50
(Size: 50 µg)
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Human Heparanase (HPA) Protein (Active) is a recombinant active Human protein.
Research Articles on Heparanase (HPA)
| Target | Heparanase (HPA) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | ELISA, WB, IP, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 97% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q9Y251 (UniProt, ExPASy) |
| UniProt Secondary AC | A9JIG7, C7F7I3, C7F7I4, E9PCA9, E9PGR1, Q53GE5, Q9UL39 |
| UniProt Entry Name | HPSE_HUMAN |
| String | 9606.ENSP00000384262 |
| Molecular Weight | Calculated MW: 17.6 kDa Observed MW (SDS-PAGE): 20 kDa |
| Sequence Fragment | Pro400-Ile543 |
| Sequence | PLPDYWLSLL FKKLVGTKVL MASVQGSKRR KLRVYLHCTN TDNPRYKEGD LTLYAINLHN VTKYLRLPYP FSNKQVDKYL LRPLGPHGLL SKSVQLNGLT LKMVDDQTLP PLMEKPLRPG SSLGLPAFSY SFFVIRNAKV AACI |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.05% Sarcosyl and 5% Trehalose. |
| Activity | Active |
| Biological Activity | Heparanase (HPA), also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides. Epithelial Cell Transforming Sequence 2 (ECT2) has been identified as an interactor of HPSE, thus a binding ELISA assay was conducted to detect the interaction of recombinant human HPSE and recombinant human ECT2. Briefly, HPSE was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to ECT2-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-HPSE polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of HPSE and ECT2 is shown in Figure 3. |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |