Human Kininogen 1 (KNG1) Protein
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Catalogue No: abx067109
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US$261.00
(Size: 10 µg)
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Datasheet SDS
Human Kininogen 1 (KNG1) / High Molecular Weight Kininogen (HMWK) is a recombinant Human protein expressed in E. coli. This protein is the immunogen for the following antibodies: abx103272
Research Articles on Kininogen 1 (KNG1)
| Target | Kininogen 1 (KNG1) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Not tested |
| Purity | > 97% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P01042 (UniProt, ExPASy) |
| UniProt Secondary AC | A8K474, B2RCR2, C9JEX1, P01043, Q53EQ0, Q6PAU9, Q7M4P1 |
| UniProt Entry Name | KNG1_HUMAN |
| KEGG | hsa:3827 |
| String | 9606.ENSP00000265023 |
| Molecular Weight | Calculated MW: 22.1 kDa Observed MW (SDS-PAGE): 26 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
| Sequence Fragment | Asp225-Thr407 |
| Sequence | DTGECT DNAYIDIQLR IASFSQNCDI YPGKDFVQPP TKICVGCPRD IPTNSPELEE TLTHTITKLN AENNATFYFK IDNVKKARVQ VVAGKKYFID FVARETTCSK ESNEELTESC ETKKLGQSLD CNAEVYVVPW EKKIYPTVNC QPLGMISLMK RPPGFSPFRS SRIGEIKEET TVSPPHT |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
| Concentration | Prior to lyophilization: 300 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |