Mouse Anti-Human IgG Antibody

Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA.Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.

Target	Human IgG
Clonality	Monoclonal
Reactivity	Human
Tested Applications	ELISA, WB
Host	Mouse
Recommended dilutions	ELISA: 1/10000, WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Human IgG was isolated from human sera and purified by chromatography.
Isotype	IgG1
Form	Liquid
Purification	Purified from ascites by Protein G chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Molecular Weight	50 kDa
Buffer	PBS, containing 0.03% sodium azide.
Concentration	1 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Human IgG

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