Human Insulin Receptor Substrate 4 (IRS4) Protein

Catalogue No: abx650527
Price: £180.00
(Size: 10 µg)
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Human IRS4 Protein is a recombinant Human protein produced in E. coli using Prokaryotic expression.

This protein is the immunogen for the following antibodies: abx131525

Target Insulin Receptor Substrate 4 (IRS4)
Origin Human
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 95%
Reconstitution Reconstitute to the original concentration in ddH2O. If further dilutions are required, dilute in PBS, pH 7.4, to a concentration of 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC O14654 (UniProt, ExPASy)
UniProt Entry Name IRS4_HUMAN
KEGG hsa:8471
String 9606.ENSP00000361202
Molecular Weight Calculated MW: 25.0 kDa
Observed MW (SDS-PAGE): 29 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Met1-Arg196
Sequence MASCSFTRDQ ATRRLRGAAA AAAAALAAVV TTPLLSSGTP TALIGTGSSC PGAMWLSTAT GSRSDSESEE EDLPVGEEVC KRGYLRKQKH GHRRYFVLKL ETADAPARLE YYENARKFRH SVRAAAAAAA AAASGAAIPP LIPPRRVITL YQCFSVSQRA DARYRHLIAL FTQDEYFAMV AENESEQESW YLLLSR
Tag N-terminal His tag
Buffer Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
Activity Not tested
Concentration Prior to lyophilization: 200 µg/ml
Availability Shipped within 5-7 working days.
Note This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Research Articles on Insulin Receptor Substrate 4 (IRS4)

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