Human Methyl CpG Binding Protein 2 (MECP2) Protein
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Catalogue No: abx650076
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(Size: 10 µg)
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Datasheet SDS
Human MECP2 Protein is a recombinant Human protein expressed in E. coli. This protein is the immunogen for the following antibodies: abx131551, abx173560
Research Articles on Methyl CpG Binding Protein 2 (MECP2)
| Target | Methyl CpG Binding Protein 2 (MECP2) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Not tested |
| Purity | > 90% |
| Reconstitution | Reconstitute in ddH2O to a concentration between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P51608 (UniProt, ExPASy) |
| UniProt Secondary AC | O15233, Q6QHH9, Q7Z384 |
| UniProt Entry Name | MECP2_HUMAN |
| Gene Symbol | MECP2 |
| GeneID | 4204 |
| HGNC | 690 |
| KEGG | hsa:4204 |
| String | 9606.ENSP00000395535 |
| Molecular Weight | Calculated MW: 23.4 kDa Observed MW (SDS-PAGE): 30 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
| Sequence Fragment | Met1-Lys177 |
| Sequence | MVAGMLGLRE EKSEDQDLQG LKDKPLKFKK VKKDKKEEKE GKHEPVQPSA HHSAEPAEAG KAETSEGSGS APAVPEASAS PKQRRSIIRD RGPMYDDPTL PEGWTRKLKQ RKSGRSAGKY DVYLINPQGK AFRSKVELIA YFEKVGDTSL DPNDFDFTVT GRGSPSRREQ KPPKKPK |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 100 mM NaHCO3, 500 mM NaCl, pH 8.3, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300. |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |