Human Neurofilament Heavy Polypeptide (NEFH) Protein

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Catalogue No: abx654507
Price: US$232.00
(Size: 10 µg)

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Human Neurofilament Heavy Polypeptide (NEFH) Protein

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Datasheet SDS
Human Neurofilament Heavy Polypeptide (NEFH) Protein is a recombinant Human protein expressed in E. coli.

Target Neurofilament Heavy Polypeptide (NEFH)
Research Area Neuroscience
Origin Human
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Activity Not tested
Purity > 90%
Reconstitution Reconstitute in ddH2O. The stock concentration should be between 0.1-0.2 mg/ml. Do not vortex.
Storage Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.
Shelf Life 12 months.
UniProt Primary AC P12036 (UniProt, ExPASy)
UniProt Entry Name NFH_HUMAN
Gene Symbol NEFH
GeneID 4744
KEGG hsa:4744
String 9606.ENSP00000311997
Molecular Weight Calculated MW: 42.3 kDa
Observed MW: 65 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Lys671-Lys1020
Sequence KAEAKSPEKA KSPVKAEAKS PEKAKSPVKE EAKSPEKAKS PVKEEAKSPE KAKSPVKEEA KTPEKAKSPV KEEAKSPEKA KSPEKAKTLD VKSPEAKTPA KEEARSPADK FPEKAKSPVK EEVKSPEKAK SPLKEDAKAP EKEIPKKEEV KSPVKEEEKP QEVKVKEPPK KAEEEKAPAT PKTEEKKDSK KEEAPKKEAP KPKVEEKKEP AVEKPKESKV EAKKEEAEDK KKVPTPEKEA PAKVEVKEDA KPKEKTEVAK KEPDDAKAKE PSKPAEKKEA APEKKDTKEE KAKKPEEKPK TEAKAKEDDK TLSKEPSKPK AEKAEKSSST DQKDSKPPEK ATEDKAAKGK
Tag N-terminal His tag
Buffer Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.
Concentration Prior to lyophilization: 50 µg/ml
Availability Shipped within 5-7 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Neurofilament Heavy Polypeptide (NEFH)


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