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Human Parathyroid Hormone (PTH) Protein (Active)
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Catalogue No: abx655795
Price:
US$623.50
(Size: 50 µg)
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Human Parathyroid Hormone (PTH) Protein (Active) is an Active Recombinant Human protein expressed in E. coli.
Research Articles on Parathyroid Hormone (PTH)
| Target | Parathyroid Hormone (PTH) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 90% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P01270 (UniProt, ExPASy) |
| UniProt Secondary AC | Q4VB48, Q9UD38 |
| UniProt Entry Name | PTHY_HUMAN |
| Gene Symbol | PTH |
| GeneID | 5741 |
| KEGG | hsa:5741 |
| String | 9606.ENSP00000282091 |
| Molecular Weight | Calculated MW: 10.8 kDa Observed MW (SDS-PAGE): 13 kDa |
| Sequence Fragment | Arg31-Gln115 |
| Sequence | RSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
| Activity | Active |
| Biological Activity | Parathyroid hormone (PTH) is an 84-amino-acid peptide hormone that is secreted by the parathyroid gland. It is a major endocrine regulator of vitamin D metabolism. It has been identified as a potent down-regulator of Vitamin D Receptor (VDR), thus a binding ELISA assay was conducted to detect the interaction of recombinant human PTH and recombinant mouse VDR. Briefly, PTH was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to VDR-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-PTH polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of PTH and VDR is shown in the figures. |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |