Platelet-Activating Factor Acetylhydrolase (PLA2G7) Protein

Human Phospholipase A2, Lipoprotein Associated (PLA2G7) Protein is an Active recombinant Human protein. This protein exhibits binding activity as detailed in the Biological Activity section below. Other types of biological activity, such as enzymatic activity, have not been tested.

Target	Platelet-Activating Factor Acetylhydrolase (PLA2G7)
Research Area	Enzymes and Kinases, Metabolic Pathways, Infection Immunity
Origin	Human
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Activity	Exhibits binding activity. Enzymatic activity has not been tested.
Biological Activity	A binding ELISA assay was conducted to detect the interaction of recombinant Human LpPLA2 and recombinant rat PON1. Briefly, PLA2G7 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PON1-coated microplate wells and incubated for 1 h at 37 °C. Wells were washed with PBST and incubated for 1 h with anti-PLA2G7 polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 5 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of recombinant human PLA2G7 and recombinant rat PON1 is shown in the figures. The EC50 for this effect is 0.7 µg/ml.
Purity	> 90%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH₂O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.
Shelf Life	12 months.
UniProt Primary AC	Q13093 (UniProt, ExPASy)
UniProt Secondary AC	A5HTT5, Q15692, Q5VTT1, Q8IVA2
UniProt Entry Name	PAFA_HUMAN
Gene Symbol	PLA2G7
GeneID	7941
OMIM	147050
HGNC	9040
KEGG	hsa:7941
Molecular Weight	Calculated MW: 49.0 kDa Observed MW (SDS-PAGE): 50 kDa
Sequence Fragment	Phe22-Asn441
Tag	N-terminal His tag
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.
Endotoxin Level	< 1.0 EU/µg protein (LAL method)
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Research Articles on Platelet-Activating Factor Acetylhydrolase (PLA2G7)

Human Platelet-Activating Factor Acetylhydrolase (PLA2G7) Protein

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