Human DNA-Directed RNA Polymerase II Subunit RPB2 (POLR2B) ELISA Kit

Human DNA-Directed RNA Polymerase II Subunit RPB2 (POLR2B) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human DNA-Directed RNA Polymerase II Subunit RPB2 (POLR2B) concentrations in tissue homogenates, cell lysates and other biological fluids. This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014].

Target	DNA-Directed RNA Polymerase II Subunit RPB2 (POLR2B)
Reactivity	Human
Tested Applications	ELISA
Recommended dilutions	Optimal dilutions/concentrations should be determined by the end user.
Storage	Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity	The validity for this kit is 6 months.
Stability	The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
UniProt Primary AC	P30876 (UniProt, ExPASy)
UniProt Secondary AC	A8K1A8, Q8IZ61
UniProt Entry Name	RPB2_HUMAN
Gene Symbol	POLR2B
GeneID	5431
OMIM	180661
HGNC	9188
KEGG	hsa:5431
Ensembl	ENSG00000047315
String	9606.ENSP00000370625
Test Range	0.156 ng/ml - 10 ng/ml
Sensitivity	< 0.08 ng/ml
Standard Form	Lyophilized
Detection Method	Colorimetric
Assay Type	Sandwich
Assay Data	Quantitative
Sample Type	Tissue homogenates, cell lysates and other biological fluids.
Availability	Shipped within 5-15 working days.
Note	This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.

Research Articles on DNA-Directed RNA Polymerase II Subunit RPB2 (POLR2B)

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