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Human Sex Hormone Binding Globulin (SHBG) Protein (Active)
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Catalogue No: abx655825
Price:
US$971.50
(Size: 50 µg)
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Human Sex Hormone Binding Globulin (SHBG) Protein (Active) is an Active Recombinant Human protein expressed in 293F cells.
Research Articles on Sex Hormone Binding Globulin (SHBG)
| Target | Sex Hormone Binding Globulin (SHBG) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | 293F cell |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 99% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.6. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.6, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P04278 (UniProt, ExPASy) |
| UniProt Entry Name | SHBG_HUMAN |
| Gene Symbol | SHBG |
| GeneID | 6462 |
| OMIM | 182205 |
| HGNC | 10839 |
| KEGG | hsa:6462 |
| Ensembl | ENSG00000129214 |
| String | 9606.ENSP00000369816 |
| Molecular Weight | Calculated MW: 42.0 kDa Observed MW (SDS-PAGE): 50 kDa |
| Sequence Fragment | Arg31-Ser401 |
| Sequence | RPVLPTQSAH DPPAVHLSNG PGQEPIAVMT FDLTKITKTS SSFEVRTWDP EGVIFYGDTN PKDDWFMLGL RDGRPEIQLH NHWAQLTVGA GPRLDDGRWH QVEVKMEGDS VLLEVDGEEV LRLRQVSGPL TSKRHPIMRI ALGGLLFPAS NLRLPLVPAL DGCLRRDSWL DKQAEISASA PTSLRSCDVE SNPGIFLPPG TQAEFNLRDI PQPHAEPWAF SLDLGLKQAA GSGHLLALGT PENPSWLSLH LQDQKVVLSS GSGPGLDLPL VLGLPLQLKL SMSRVVLSQG SKMKALALPP LGLAPLLNLW AKPQGRLFLG ALPGEDSSTS FCLNGLWAQG QRLDVDQALN RSHEIWTHSC PQSPGNGTDA S |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 10 mM PBS, pH 7.6, containing 5% Trehalose. |
| Activity | Active |
| Biological Activity | Sex Hormone-Binding Globulin (SHBG), also known as Sex Steroid-Binding Globulin (SSBG), is a glycoprotein that binds to the sex hormones estrogen, dihydrotesterone (DHT) and testerone. SHBG transports these hormones in blood to tissues in the body. A binding ELISA assay was conducted to detect the interaction of recombinant human SHBG and BSA-conjugated Testosterone. Briefly, biotin-conjugated SHBG was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to BSA-conjugated Testosterone-coated microplate wells and incubated for 1 h at 37°C. Wells were washed with PBST 3 times and incubated for 30 min with HRP-conjugate. Wells were aspirated and washed 5 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of SHBG and Testosterone is shown in Figure 4. |
| Endotoxin Level | < 1.0 EU/µg (LAL method) |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 1-2 months. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |