Human Sirtuin 3 (SIRT3) Protein

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Catalogue No: abx655827
Price: US$609.00
(Size: 50 µg)

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Datasheet SDS
Human Sirtuin 3 (SIRT3) Protein (Active) is an Active Recombinant Human protein.

Target Sirtuin 3 (SIRT3)
Origin Human
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 92%
Reconstitution Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q9NTG7 (UniProt, ExPASy)
UniProt Secondary AC B7Z5U6, Q9Y6E8
UniProt Entry Name SIR3_HUMAN
String 9606.ENSP00000372191
Molecular Weight Calculated MW: 31.7 kDa
Observed MW (SDS-PAGE): 32 kDa
Sequence Fragment Gln126-Lys399
Sequence QDVAE LIRARACQRV VVMVGAGIST PSGIPDFRSP GSGLYSNLQQ YDLPYPEAIF ELPFFFHNPK PFFTLAKELY PGNYKPNVTH YFLRLLHDKG LLLRLYTQNI DGLERVSGIP ASKLVEAHGT FASATCTVCQ RPFPGEDIRA DVMADRVPRC PVCTGVVKPD IVFFGEPLPQ RFLLHVVDFP MADLLLILGT SLEVEPFASL TEAVRSSVPR LLINRDLVGP LAWHPRSRDV AQLGDVVHGV ESLVELLGWT EEMRDLVQRE TGKLDGPDK
Tag N-terminal His tag
Buffer Prior to lyophilization: 100 mM NaHCO3, 500 mM NaCl, pH 8.3, containing 0.01% sarcosyl, 5% trehalose.
Activity Active
Biological Activity Sirtuin 3 (SIRT3), an NAD-dependent deacetylase, is a member of the mammalian sirtuin family of proteins. In humans, sirtuins have a range of molecular functions and have emerged as important proteins in ageing, stress resistance and metabolic regulation. It also can regulate epigenetic gene silencing and suppress recombination of rDNA in yeast. SIRT3 is expressed in white and brown adipose tissue. Isocitrate Dehydrogenase 2, mitochondrial (IDH2) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SIRT3 and recombinant human IDH2. Briefly, SIRT3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to IDH2-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-SIRT3 polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of SIRT3 and IDH2 is shown in Figure 3.
Concentration Prior to lyophilization: 200 µg/ml
Availability Shipped within 5-7 working days.
Note This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Research Articles on Sirtuin 3 (SIRT3)


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