Human Anti-Thyroxine Antibody (Anti-T4) ELISA Kit

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Catalogue No: abx051601
Price: US$681.50
(Size: 96 tests)

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Graph showing standard OD data for Human Anti-Thyroxine Antibody (Anti-T4)
Human Anti-Thyroxine Antibody (Anti-T4) ELISA Kit

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Datasheet SDS Download Manual
Human Anti-Thyroxine Antibody (Anti-T4) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human Anti-Thyroxine Antibody (Anti-T4) concentrations in serum, plasma and other biological fluids.

Introduction Anti-thyroxine antibodies (Anti-T4) are autoantibodies that target the thyroid. These have been documented to occur in Hashimoto’s thyroiditis, systemic lupus erythematosus, and other thyroid-related autoimmune disorders. Thyroxine is one of the major hormones released by the thyroid into the blood, and have a wide range of effects relating to metabolism. Anti-T4 contributes to hypothyroidism and may pose issues to a developing fetus if the patient is pregnant.
Target Anti-Thyroxine Antibody (Anti-T4)
Reactivity Human
Tested Applications ELISA
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Storage Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity The validity for this kit is at least 6 months. Up to 12 months validity can be provided on request.
Stability The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
UNSPSC Code 41116158
Test Range 1.02 ng/ml - 65 ng/ml
Sensitivity 0.61 ng/ml
Standard Form Lyophilized
Detection Method Colorimetric
Assay Type Indirect
Assay Data Quantitative
Sample Type Serum, plasma and other biological fluids.
Kit Components The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
  • Pre-coated 96-Well Microplate
  • Standard
  • Standard Diluent Buffer
  • Wash Buffer
  • Detection Reagent A
  • Detection Reagent B
  • Diluent A
  • Diluent B
  • TMB Substrate
  • Stop Solution
  • Plate Sealer
Material Required But Not Provided
  • 37°C incubator
  • Multi and single channel pipettes and sterile pipette tips
  • Squirt bottle or automated microplate washer
  • 1.5 ml tubes
  • Distilled water
  • Absorbent filter papers
  • 100 ml and 1 liter graduated cylinders
  • Microplate reader (wavelength: 450 nm)
  • ELISA Shaker
Assay Procedure This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
  • 1) Set standard, test samples and control wells.
  • 2) Aliquot 100 µl of diluted standard into the standard wells.
  • 3) Aliquot 100 µl of Standard Diluent buffer into control (zero) well.
  • 4) Aliquot 100 µl of diluted samples into the sample wells. Incubate for 1 hr at 37 °C.
  • 5) Aliquot 100 µl of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
  • 6) Wash 3 times.
  • 7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 90 mins at 37 °C.
  • 8) Wash 5 times.
  • 9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
  • 10) Aliquot 50 µl of Stop Solution.
  • 11) Measure the OD at 450 nm.
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Anti-Thyroxine Antibody (Anti-T4) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Anti-Thyroxine Antibody (Anti-T4) were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) × 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%
Availability Shipped within 5-7 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.

Please note that our kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
Plate coated with Antigen
Research Articles on Anti-Thyroxine Antibody (Anti-T4)


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