Human DNA Topoisomerase I (TOP1) Protein
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Catalogue No: abx069378
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US$261.00
(Size: 10 µg)
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Datasheet SDS
Human DNA Topoisomerase I (TOP1) is a recombinant Human protein expressed in E. coli.
Research Articles on DNA Topoisomerase I (TOP1)
| Target | DNA Topoisomerase I (TOP1) |
| Research Area | Enzymes and Kinases, Developmental Science, Cancer |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Not tested |
| Purity | > 80% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P11387 (UniProt, ExPASy) |
| UniProt Entry Name | TOP1_HUMAN |
| Gene Symbol | TOP1 |
| GeneID | 7150 |
| OMIM | 126420 |
| NCBI Accession | NP_003277.1 |
| HGNC | 11986 |
| KEGG | hsa:7150 |
| Ensembl | ENSG00000198900 |
| String | 9606.ENSP00000354522 |
| Molecular Weight | Calculated MW: 33.9 kDa Observed MW (SDS-PAGE): 38 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
| Sequence Fragment | Met1-Ser250 |
| Sequence | MSGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK EKDREKSKHS NSEHKDSEKK HKEKEKTKHK DGSSEKHKDK HKDRDKEKRK EEKVRASGDA KIKKEKENGF SSPPQIKDEP EDDGYFVPPK EDIKPLKRPR DEDDADYKPK KIKTEDTKKE KKRKLEEEED GKLKKPKNKD KDKKVPEPDN KKKKPKKEEE QKWKWWEEER YPEGIKWKFL EHKGPVFAPP YEPLPENVKF YYDGKVMKLS |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl and 5% Trehalose. |
| Concentration | Prior to lyophilization: 500 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |