Transforming Growth Factor Beta Receptor II (TGFbR2) Protein

Human Transforming Growth Factor Beta Receptor II (TGFbR2) Protein is an active recombinant Human protein expressed in E. coli. This protein exhibits binding activity as detailed in the Biological Activity section below. Other types of biological activity, such as enzymatic activity, have not been tested.

Target	Transforming Growth Factor Beta Receptor II (TGFbR2)
Research Area	Infection Immunity
Origin	Human
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Activity	Active
Biological Activity	Transforming growth factor beta receptor 2 (TGFBR2) is a tumor suppressor gene that plays a role in the differentiation of striated cells and remodeling of coronary arteries. Single nucleotide polymorphisms (SNPs) of this gene are associated with Marfan syndrome and sudden death in patients with coronary artery disease. Cardiovascular remodeling and T cell activation of TGFBR2 gene suggest that the TGFBR2 gene SNPs are related to the pathogenesis of Kawasaki disease (KD) and coronary artery lesion (CAL). Endoglin can regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis. A functional ELISA assay was conducted to detect the interaction of recombinant human TGFBR2 and recombinant rat Endoglin (ENG). Briefly, TGFBR2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ENG-coated microplate wells and incubated for 1 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-TGFBR2 polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody for 1 h at 37°C, wells were aspirated and washed 5 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450/630 nm immediately. The binding activity of recombinant human TGFBR2 and recombinant rat ENG is shown in Figure 2. The EC50 is 36.1 ng/ml.
Purity	> 95%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH₂O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.
Shelf Life	12 months.
UniProt Primary AC	P37173 (UniProt, ExPASy)
UniProt Secondary AC	B4DTV5, Q15580, Q6DKT6, Q99474
UniProt Entry Name	TGFR2_HUMAN
Gene Symbol	TGFBR2
GeneID	7048
KEGG	hsa:7048
String	9606.ENSP00000351905
Molecular Weight	Calculated MW: 20.0 kDa Observed MW (SDS-PAGE): 22 kDa
Sequence Fragment	Thr23-Gln166
Sequence	TIPPHVQK SVNNDMIVTD NNGAVKFPQL CKFCDVRFST CDNQKSCMSN CSITSICEKP QEVCVAVWRK NDENITLETV CHDPKLPYHD FILEDAASPK CIMKEKKKPG ETFFMCSCSS DECNDNIIFS EEYNTSNPDL LLVIFQ
Tag	N-terminal His tag
Buffer	Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.
Endotoxin Level	< 1.0 EU/µg protein (LAL method)
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Research Articles on Transforming Growth Factor Beta Receptor II (TGFbR2)

Human Transforming Growth Factor Beta Receptor II (TGFbR2) Protein (Active)

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