Checkpoint Protein HUS1 (HUS1) Antibody

HUS1 Antibody is a Rabbit Polyclonal antibody against HUS1. The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.

Target	Checkpoint Protein HUS1 (HUS1)
Clonality	Polyclonal
Reactivity	Human, Mouse
Tested Applications	WB, IF/ICC, IP
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/2000, IF/ICC: 1/50 - 1/100, IP: 0.5-4 µg/200-400 µg whole cell extracts. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HUS1.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	O60921 (UniProt, ExPASy)
UniProt Secondary AC	B4DFI9
UniProt Entry Name	HUS1_HUMAN
Gene Symbol	HUS1
GeneID	3364
NCBI Accession	NP_004498.1
KEGG	hsa:3364
String	9606.ENSP00000258774
Molecular Weight	Calculated MW: 32 kDa Observed MW: 35 kDa
Buffer	PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Checkpoint Protein HUS1 (HUS1)

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