DNA-Binding Protein Ikaros (IKZF1) Antibody

DNA-binding protein Ikaros (IKZF1) Antibody is a Rabbit Polyclonal antibody against DNA-binding protein Ikaros (IKZF1). This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL).

Target	DNA-Binding Protein Ikaros (IKZF1)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	WB, IF/ICC
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/1000, IF/ICC: 1/50 - 1/200, ChIP: 5 µg/10-15 µg Chromatin. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-270 of human Ikaros.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q13422 (UniProt, ExPASy)
UniProt Secondary AC	A4D260, B4E0Z1, D3DVM5, O00598, Q53XL2, Q69BM4, Q8WVA3
UniProt Entry Name	IKZF1_HUMAN
Gene Symbol	IKZF1
GeneID	10320
NCBI Accession	NP_001207694.1
KEGG	hsa:10320
String	9606.ENSP00000331614
Molecular Weight	Calculated MW: 58 kDa Observed MW: 50-70 kDa
Buffer	PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on DNA-Binding Protein Ikaros (IKZF1)

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