Human Interleukin 17C (IL17C) Protein (Active)

Human Interleukin 17C (IL17C) Protein (Active) is an active Human protein expressed in E. coli. This protein exhibits binding activity as detailed in the Biological Activity section below. Other types of biological activity, such as enzymatic activity, have not been tested.

Target	Interleukin 17C (IL17C)
Research Area	Cytokines
Origin	Human
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Activity	Active
Biological Activity	Interleukin 17C (IL17C) is a T cell-derived cytokine which has high sequence homology with Interleukin 17 (IL17) and may play a crucial role in innate immunity of the epithelium. IL17C binds to Interleukin 17 receptor A (IL17RA), thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL17C and recombinant human IL17RA. Briefly, IL17C was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to IL17RA-coated microplate wells and incubated for 2 h at 37 °C. Wells were washed with PBST and incubated for 1 h with anti-IL17C polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of IL17C and IL17RA is shown in Figure 3.
Purity	> 95%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH₂O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.
Shelf Life	12 months.
UniProt Primary AC	Q9P0M4 (UniProt, ExPASy)
UniProt Secondary AC	Q3MIG8, Q9HC75
UniProt Entry Name	IL17C_HUMAN
String	9606.ENSP00000244241
Molecular Weight	Calculated MW: 21.0 kDa Observed MW (SDS-PAGE): 25 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment	MG + His19-Val197 + LEHHHHHH
Sequence	MG-HH DPSLRGHPHS HGTPHCYSAE ELPLGQAPPH LLARGAKWGQ ALPVALVSSL EAASHRGRHE RPSATTQCPV LRPEEVLEAD THQRSISPWR YRVDTDEDRY PQKLAFAECL CRGCIDARTG RETAALNSVR LLQSLLVLRR RPCSRDGSGL PTPGAFAFHT EFIHVPVGCT CVLPRSV-LEHHHHHH
Tag	C-terminal His tag
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Endotoxin Level	< 1.0 EU/µg protein (LAL method)
Concentration	Prior to lyophilization: 100 µg/ml
Availability	Shipped within 5-7 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Research Articles on Interleukin 17C (IL17C)

Human Interleukin 17C (IL17C) Protein (Active)

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