Isopropyl-beta-D-thiogalactopyranoside (IPTG) is a chemical analogue of galactose, which cannot be hydrolysed by the enzyme beta-Galactosidase. It induces the E. coli lac operon activity by binding and inhibiting the lac repressor without being degraded. Genes controlled by the lac or tac promoter/operator sequences are expressed to high levels in the presence of IPTG. Melting point: 112-113 °C.

Target	IPTG
Recommended dilutions	For blue/white screening, a 0.1 mM final IPTG concentration in Luria Broth (LB) media is recommended.
Form	Solid
Purity	≥ 99% (HPLC)
Reconstitution	Reconstitute in DNase-free and RNase-free water (18.2 MΩ) to the desired concentration, then filter through a 0.22 µm filter.
Storage	Store at -20°C. Avoid repeated freeze/thaw cycles.
Shelf Life	Up to 12 months.
Molecular Weight	238.3 g/mol
Molecular Formula	C9H18O5S
Buffer	Not applicable.
CAS Number	367-93-1
Availability	Shipped within 10-20 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Research Articles on IPTG

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