Interferon Regulatory Factor 3 (IRF3) Antibody

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Catalogue No: abx001784
Price: US$217.50
(Size: 20 µl)

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Western blot analysis of lysates from C6 cells using IRF3 Antibody at 1/1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
Interferon Regulatory Factor 3 (IRF3) Antibody Interferon Regulatory Factor 3 (IRF3) Antibody Interferon Regulatory Factor 3 (IRF3) Antibody Interferon Regulatory Factor 3 (IRF3) Antibody Interferon Regulatory Factor 3 (IRF3) Antibody Interferon Regulatory Factor 3 (IRF3) Antibody

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Datasheet SDS
IRF3 Antibody is a Rabbit Polyclonal antibody against IRF3. This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Target Interferon Regulatory Factor 3 (IRF3)
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IHC, IF/ICC, IP
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/500 - 1/1000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200, IP: 0.5 µg - 4 µg antibody per 200 µg - 400 µg extracts of whole cells. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human IRF3.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q14653 (UniProt, ExPASy)
UniProt Secondary AC A8K7L2, B2RAZ3, Q5FBY1, Q5FBY2, Q5FBY4, Q7Z5G6
UniProt Entry Name IRF3_HUMAN
Gene Symbol IRF3
GeneID 3661
NCBI Accession NP_001184052.1
KEGG hsa:3661
String 9606.ENSP00000471896
Molecular Weight Calculated MW: 47 kDa
Observed MW: 45-55 kDa
Buffer PBS, pH 7.3, containing 0.09% sodium azide, 50% glycerol.
Concentration 1.76 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Interferon Regulatory Factor 3 (IRF3)


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