Ubiquitin-Like Protein ISG15 (ISG15) Antibody

ISG15 is secreted from monocytes in response to type I interferons and causes natural killer (NK) cell proliferation and an augmentation of non-MCH (major histocompatibility complex) restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted also by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated with alterations in ISG15 expression. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog.

Target	Ubiquitin-Like Protein ISG15 (ISG15)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 31-61 amino acids from the N-terminal region of human ISG15.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P05161 (UniProt, ExPASy)
UniProt Secondary AC	Q5SVA4, Q7Z2G2, Q96GF0
UniProt Entry Name	ISG15_HUMAN
NCBI Accession	NP_005092.1
KEGG	hsa:9636
String	9606.ENSP00000368699
Molecular Weight	Calculated MW: 17.9 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Ubiquitin-Like Protein ISG15 (ISG15)

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