Lysine-Specific Demethylase 3B (JMJD1B) Antibody

Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe (II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

Target	Lysine-Specific Demethylase 3B (JMJD1B)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB
Host	Rabbit
Recommended dilutions	WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 25-57 amino acids from the N-terminal region of human JMJD1B.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q7LBC6 (UniProt, ExPASy)
UniProt Secondary AC	A6H8X7, Q9BVH6, Q9BW93, Q9BZ52, Q9NYF4, Q9UPS0
UniProt Entry Name	KDM3B_HUMAN
KEGG	hsa:51780
String	9606.ENSP00000326563
Molecular Weight	Calculated MW: 192 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse KDM3B.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Lysine-Specific Demethylase 3B (JMJD1B)

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