KD Plus DNA Polymerase Enzyme (with 2.5 mM dNTPs)

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Catalogue No: abx071023
Price: US$362.50
(Size: 100 U)
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KD Plus DNA Polymerase Enzyme (with 2.5 mM dNTPs)

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Documents

Datasheet SDS
KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase.

Contents:

Component 100 U 200 U 1200 U
KD Plus DNA Polymerase100 U 200 U 6 × 200 U
5X KD Plus Buffer 1.2 ml 2 × 1.2 ml 12 × 1.2 ml
2.5 mM dNTPs 500 µl 1 ml 6 × 1 ml
50 mM MgSO4 200 µl 400 µl 1 ml
6X DNA Loading Buffer 500 µl 1 ml 2 × 1 ml


Target KD Plus DNA Polymerase
Tested Applications PCR
Conjugation Unconjugated
Storage Store at -20°C. Avoid repeated freeze/thaw cycles.
Buffer Enzyme Buffer: 50 mM Tris-Cl (pH 8.0), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, Stabilizers, 50% glycerol.
KD Plus Buffer with 5 mM MgSO4: Contains 100 mM Tris-Cl (pH 9.2), 50 mM NH4SO4, 200 mM KCl, 5 mM MgSO4 and 10% glycerol.
Availability Shipped within 10-20 working days.
Note This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Directions for use Reaction Components:

Component Volume Final Concentration
Template Variable as required
Forward Primer (10 µM) 1 µl 0.2 µM
Reverse Primer (10 µM) 1 µl 0.2 µM
5X KD Plus Buffer 10 µl 1X
2.5 mM dNTPs 4 µl 0.2 mM
KD Plus DNA Polymerase 0.1 µl 1 U
Nuclease-free H2O Variable N/A
Total Volume 50 µl N/A

Thermal Cycling Conditions:

Number of Cycles Temperature Time
1 cycle 94 °C 2-5 min
30-25 cycles 94 °C 30 seconds
50-60 °C 30 seconds
72 °C 1 kb/min
1 cycle 72 °C5-10 min
Research Articles on KD Plus DNA Polymerase


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